Figure 6: Actin cytoskeletal morphology and actin expression in RAW 264.7 and Maf-DKO cells. Western blot quantification of actin expression in RAW 264.7 and Maf-DKO cells relative to tubulin (A). Data represent ratios of actin and tubulin band intensities of one blot. B-E, Cells were seeded on glass coverslips and stimulated overnight with LPS (100 ng/ml) or left unstimulated. After fixation, cells were stained with Alexa568- labled phaloidin and imaged on a Zeiss LSM510 META confocal laser scanning microscope.