Figure 7: Phagocytosis of COZ in RAW 264.7 and Maf-DKO cells in the presence and absence of M-CSF, LPS, or metabolic inhibitors. A, Phagocytic index of RAW 264.7 and Maf-DKO cells was determined with or without LPS (±LPS) and with or without L929-cell CM (± M-CSF). All bars were normalized to the RAW 264.7+LPS bar. Data represent means ± SEM of three independent experiments performed in triplo. (*p<0.05, **p<0.01, ***p<0.001, two-way ANOVA and Bonferroni posttest). Phagocytosis capacity in the presence of OXPHOS inhibitor oligomycin (2.5 μM) (B), or glycolysis inhibitor 2-DG (10 mM) (C). Inhibitors were administered for the time periods indicated. Analysis of data was as given in Venter et al. [46]. Figures represent normalized means ±SEM of three independent experiments performed in triplicate. (*p<0.05, **p<0.01, ***p<0.001; one-sample t-test). Note the fluctuations in phagocytic index in RAW 264.7 cells, caused by oligomycin administration. At 24 hour oligomycin inhibition consistently produced even a stimulatory effect. We attribute these variations to metabolic adaptation to medium replenishment, for which the RAW 264.7 lineage appears highly sensitive