Figure 5: Detection of sialic acids at the cell surface of differentiated macrophages. On day 6, macrophages were treated or not with DAS181 at 250U/mL in RPMI 1640 medium. On day 7, cells were collected by scraping and subjected to lectin staining. Biotinylated MAL II and FITC-SNA was incubated with cells in TBS. PEconjugated streptavidin was used for revealing MAL II binding. The x-axis shows arbitrary unit of fluorescent signal and y-axis shows percentage of maximum cell numbers. The lines are representative profile of 3 donors.