Figure 6: Secretion of cytokine and chemokine in the supernatants of polarized macrophages after mock and influenza infection. Differentiated primary human macrophages were infected with H1N1, H9N2 or H5N1 at a MOI of 2. Mock infected cells served as controls. At 6 and 24h p.i., supernatants of culture were collected and analysed by cytokine bead assay to estimate the expression of IL6, IL10, CXCL10, CCL3 and TNFα.