Selected
Figure 3: Selected cytokine levels of PC-3 High Invasion cells, DU145 and U-937 and co-culture supernatants. The supernatant cytokine levelswere assayed by ELISA after 48 hours culture. (A) Co-cultures of U-937 cells with PC-3 High Invasion or DU145 cells yield increased CCL2levels compared with cancer cell or U-937 supernatants alone. (B) High Gro-alpha levels were detected in PC-3 cell cultures and weredecreased in the co-cultures and not quite statistically different, P=0.0561. (C). High Interleukin-6 levels were detected in PC-3 High Invasioncell cultures and were not significantly different in the co-cultures. (D) PC-3 High Invasion cell cultures yielded high Interleukin-8 levelswhich were not significantly different in the co-cultures. DU-145 yielded low Gro-alpha, IL-6 and IL-8 levels. The data are expressed as mean± SD of four independent experiments. *P<0.01, **P<0.001.