Figure 7: Prostate cancer cell invasion and NF-κB activity in co-cultures. (A) The percent nuclear NF-κB was significantly induced in PC-3High Invasive prostate cancer cells co-cultured with U-937 monocytes versus alone. (B) Relative PC-3 invasion in co-culture with U-937 cellswas reduced from 196 ± 7 to 151 ± 12 percent by transfection with dominant negative IκBαS32/36A versus control vector. (C) Dosedependent nuclear NF-κB DNA binding activity was induced in PC-3 High Invasive cells following incubation with recombinant humanCCL2 for 24 hours. (D) The CCL2 induced PC-3 High Invasive cell NF-κB activity was reduced when the cells were treated with anti-CCL2neutralizing antibodies. The data are expressed as the mean ± SD of three independent experiments. *P<0.05, **P<0.01.