Figure 7: Electrophoretic mobility shift assay of RA IgG with native and nitroxidized-albumin on SDS-polyacrylamide gel. Electrophoresis was carried out on 7.5% SDS-PAGE for 3.5 h at 70 V. (A) native albumin (10 μg, lane 1) and (B) nitroxidized-albumin (1.5 mM peroxynitrite) ( 10 μg, lane 1) were incubated with 10, 20, 30 and 40 μg RA IgG (lane 2-5) at 37ᵒC for 2 h and overnight at 4°C.