Figure 4: IL-4 expressing tumors contain evidence of M2-polarized myeloid cells, reduced proliferation and increased apoptosis. EVAC2M2 or IL4-AC2M2 tumors were resected at 500 mm3 volumes from engrafted Rag2-/-; IL2Rγc-/- mice, fixed with paraformaldehyde and embedded in paraffin (n=3 for each cohort). Five μm sections were stained using an automated Ventana system with the indicated antibodies. Quantitation was carried out using Aperio image analysis software. A: CD11b, P=0.1449; B: arginase I, P=0.0012; C: cleaved caspase 3, P=0.0451; and D: Ki67, P=0.0244.