Figure 5: Heat map of genes with significant changes of expression in IL4 vs EV tumors. In biological triplicates, 100 ng of total tumor RNA was assayed using the nCounter Mouse Immunology CodeSet with added custom genes according to manufacturer protocol. Results were normalized and analyzed using NanoStriDE software package with default settings. Genes with significantly changed expression were analyzed with hierarchical clustering by Euclidean distance using MultiExperiment Viewer v.49 (MeV). Data was transformed by natural logarithm and normalized to the average expression across all samples.