Figure 2: Negative cis-acting motifs and transient transfectants. (A). Schematic representation of the relative positions within the MR gene where negative cis-acting motifs were removed. (B). Transient transfection of HeLa and 293T cells was performed with gene optimized MR. Following incubation, cells were stained with monoclonal anti-MR and secondary stained with goat anti-mouse Alexa 555. Nuclear staining is achieved with ToPro-3. Images were captured with a 63x oil objective on the Zeiss LSM-510 (B) Demonstrates the distribution of MR in HeLa cells transfected with OptMR after 48 hours incubation.