Figure 5: Co-Localization of MR with cellular proteins TLR2 and Rab5. HeLa OptMR stable transfectants were incubated with approximately five pHrodo SA particles per cell for 30 minutes. Following incubation, cells were stained with monoclonal anti-MR and rabbit polyclonal anti-TLR2 (A) or Rab5a (B). Secondary staining with goat anti-mouse Alexa 488 and goat anti-rabbit 647. Images were captured with a 100x oil objective on the Zeiss LSM-510.