binding
Figure 2: SAP binding to amyloid precursor protein. (A-F) Binding of SAP to HSA and AP-HAS in the presence of 2 mM Ca2+ (A), in PBS (B) in PBS with different concentrations of Ca2+ (C), in 2 mM Ca2+ plus different concentrations of EDTA (D), or with different concentrations of Cu2+ (E) or Mg2+ (F) was assessed by ELISA. Error bars are means ± SEM of duplicate wells. Similar results were obtained from at least 2 independent experiments (*p<0.05, **p<0.005, ***p<0.0005 and ****p<0.00005 compared with HSA).