Figure 5: Misfolded structure in amyloid precursor protein is crucial for pentraxin binding. (A, B) EGCG was incubated with HSA or AP-HSA for 1 hr and binding of SAP (A) or CRP (B) to HAS or AP-HSA was assessed by dot blot. Similar results were obtained from at least two independent experiments. (C, D) Binding of SAP (C) or CRP (D) to HSA or HSA crosslinked with EDC (AP-HSA), DMP (HSA-DMP) or glutaraldehyde (HSA-Glut) was assessed by ELISA. Error bars are means ± SEM of duplicate wells. Similar results were obtained from at least two independent experiments.