Figure 6: Effect of pentraxin binding to amyloid precursor protein in cellular cytotoxicity. (A-D) Biotinylated HSA or AP-HSA (1 µg/ml) preincubated with different concentrations of SAP or CRP was added to RPMI 8226 cells. After 1 hr on ice, streptavidin- AF488 and propidium iodide were added to analyze binding of HSA or AP-HSA (A and B) and cell death (C and D) by flow cytometry. Error bars are means ± SEM of 3 pooled experiments (*p<0.05 and ***p<0.0005).