Figure 7: SAP inhibits IFN-α production triggered by DNAcontaining amyloids. (A-C) PBMC were incubated with control (HSA+DNA) or DNA-amyloid with or without SAP (50 µg/ml) for 24 hrs. Supernatants were analyzed by ELISA for IFN-α (A), IL-6 (B) and TNFα (C) secretion. Error bars are means± SEM of six donors. (D) Purified pDCs were incubated with control or DNAamyloid, and IFN-α was quantified by ELISA after 24 hrs of culture. Error bars are means ± SEM of six donors (*p<0.05 and **p<0.005 compared with DNA-amyloid without SAP).