Figure 1: p210-cBSA immunization favorably induce a regulatory T cell phenotype in spleen after immunization and in vitro incubation. C57Bl/6 mice were immunized with p210-cBSA and analyzed for Tregs in spleen one week after last immunization using flow cytometry (A) Tregs were gated as CD25+FoxP3+ cells out of CD3+CD4+ cells. Spleen cell proliferation in response to CD3/ CD28 beads activation after p210-cBSA immunization (B). The proliferative capacity of the cells is expressed as counts per minute (CPM) and mice immunized with PBS served as control (*p ≤ 0.05).