Figure 5: IL-12 release decreases after incubation with p210-cBSA and addition of IL-12 restores T cell proliferation. Release of IL-10 and IL-12 was determined after incubation of DCs, Tregs and polyclonally activated T effector cells with increasing concentrations of p210-cBSA, cBSA or p210 (A and B). IL-12 release was determined after incubation of DCs and polyclonally activated T effector cells with p210-cBSA, cBSA or p210 in the absence of Tregs (C). 100 pg/ml of IL-12 was added to selected cocultures of DCs, Tregs and polyclonally activated T cells in the presence or absence of p210-cBSA. Proliferation of cells was determined after 72 hours and is expressed as counts per minute (CPM) (D). Neutralizing IL-10 antibody was added to selected wells of DCs, Tregs and polyclonally activated T effector cells in the presence or absence of p210-cBSA or cBSA (E). (*p ≤ 0.05, **p ≤ 0,01, ***p<0.001).