Figure 1: Modulation of the expression of HLA-DR and CD86 on dendritic cells. Cells were obtained from human monocytes as it was stated in Materials and Methods. Then, cells were cultured in the absence or presence of LPS (500 ng/ml) to obtained inmature (i) or mature (m) dendritic cells (DC) respectively. DC were stimulated during 1 h with different concentrations of T1N0Mx- IgG to analyze A) HLA-DR or C) CD86 expression. Also the effect of normal-IgG on B) HLA-DR or D) CD86 expression in DC was analyzed. Markers were measured by flow cytometry. Data represent the mean fluorescence intensity ± S.E.M. (n=3). *p<0.0001 vs. control iDC; #p<0.01 and ###p<0.0001 vs. control mDC.