Maturation Marker (Fluorescence intensity units) Treatment IgG (10-8M) IgG + AT(10-8M) IgG + MM (10-7M) HLA-DR 72.5 ± 18.6 120.8 ± 18.0*** 77.7 ± 19.8 CD86 497.4 ± 26.3 655.9 ± 42.6** 490.2 ± 29.9 Mature dendritic cells were obtained by culturing cells with LPS (500 ng/ml) during 24 h at 37°C. Then cells were treated during 1 hr with IgG from breast cancer patients in T1N0Mx in the absence or presence of the muscarinic antagonist atropine (AT) or the nicotinic antagonist mecamylamine (MM) added 30 min before IgG. The expression of HLA-DR and CD86 was measured by flow cytometry. Data represent the mean±S.E.M of 4 experiments. **p£0.001; ***p£0.0001 vs. IgG.

Table 1: Effect of cholinergic antagonists on the IgG-modulated expression of maturation markers in mature dendritic cells.