Cytokine (pg/ml) Treatment
IgG (10-8M) IgG + AT (10-8M) IgG + MM (10-7M)
TNF-α 2023.3 ± 494.1 3743.2 ± 449.5* 2632.4 ± 301.6
IL-12 761.1 ± 68 623.3 ± 134.4 417.4 ± 109.9**
IL-10 376.1 ± 65.6 318.3 ± 44.1 193.5 ± 19.1**
Mature dendritic cells were obtained by culturing cells with LPS (500 ng/ml) during 24 h at 37°C. Then cells were treated during 1 h with IgG from breast cancer patients in T1N0Mx in the absence or presence of the muscarinic antagonist atropine (AT) or the nicotinic antagonist mecamylamine (MM) added 30 min before IgG. The production of cytokines was measured in cell culture supernatants by ELISA. Data represent the mean±S.E.M. of 4 experiments. *p£0.01; **p£0.001 vs. IgG.
Table 2: Effect of cholinergic antagonists on the IgG-modulated production of cytokines by mature dendritic cells.