Figure 1: Comparison of the percentages of blast cells among PBMC induced by mycobacterial antigens in healthy controls (CTRL, n=12), LTBI subjects (LTBI, n=28) and TB patients (TB, n=17). Panel A and B represent frequencies of CD3+CD4+ (A) and CD3+CD8+ (B) expanded T-lymphoblasts generated after longterm stimulation of PBMC with HBHA. Panel C and D represent frequencies of ESAT-6 expanded CD3+CD4+ (C) and CD3+CD8+ (D) T lymphoblasts. Frequencies of blasts obtained in unstimulated cultures were subtracted. Horizontal lines represent median frequencies. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.