Figure 2: Representative FACS plots of total T-cell functionality representative FACS plots of CD4+ and CD8+ T cells in patients without EBV reactivation (two left columns), low EBV reactivation (two center columns) and high EBV reactivation (two right columns) throughout follow up (t=0, 2, 4, 6 and 12). IFNγ production was measured after restimulation with PMA/ionomycin following 12 day stimulation with BZLF-1 peptide pool. FACS plots show CD4+ (left panel for each patient) or CD8+ (right panel for each patient) expression on x-axis and IFNγ production on y-axis. Percentages in upper right quadrant indicate the percentage of IFNγ producing CD4+ or CD8+ T cells.