Figure 2: iNOS levels are modulated by miRNA gain or loss of function. iNOS mRNA (A) and protein levels (B) decreased in miR-323-5p mimic-transfection group and increased in miR-323-5p-inhibitor transfection group. (C) The iNOS 3′UTRluciferase construct was co-transfected in X-ALD patient-derived lymphocytes with miR-323-5p mimic or inhibitor or negative control (NGC) miRNA. Relative renilla luciferase activity is expressed as mean ± SD. Data are represented as the mean ± SD of three different experiments. Luciferase activity in mimic (MIM) and inhibitor (INB) were compared to NGC-transfected cells. ALD-C was compared to healthy controls (CTL). *p<0.05, **p<0.01, ***p<0.001. CTL: healthy control lymphocytes; ALD: X-ALD patient-derived lymphocytes.