Figure 2: Overexpression of miR-24 inhibits the secretion of TNF-α, IL-6 and IL-12(p40) (measured by ELISA) by macrophages stimulated with Aa-, Pg-, and Pg CSE-derived LPS. A) TNF-α levels in the culture supernatant of macrophages transfected with miR-24 mimic, inhibitor, or con-trol mimic (all at 50 nM), or positive control (untransfected) for 18 h, and stimulated with Aa-, Pg-, or Pg CSE-LPS (all at 100 ng/ml) was assayed by ELISA at 18 and 72 h of stimulation. B) IL-6 levels assayed by ELISA. C) IL-12(p40) levels assayed by ELISA. A, B, C = Mean values, error bars (standard deviation), and significance calculated by ANOVA). n=4 biological donors. (*P<0.05, **P<0.001).