Figure 3: Macrophage activation state modulates the inhibitory capacity of miR-24 on Aa-LPS in-duced cytokine secretion. A) TNF-α levels in the culture supernatant of macrophages transfected with miR-24 mimic, inhibitor, control mimic (all at 50 nM), or positive control (untransfected) for 18 h, primed with IFN-γ (10 ng/ml), IFN-γ plus TNF-α (1 ng/ml), IFN-γ+TNF-β (1 ng/ml), IFN-γ plus IL-17A (10 ng/ml), or positive control (no cytokine priming) for 18 h, and stimulated with Aa-LPS (100 ng/ml) for 12 h, was assayed by ELISA. B) IL-6 levels assayed by ELISA. C) IL-12(p40) levels assayed by ELISA. A, B, C=Mean values, error bars (standard deviation), and significance calculated by ANOVA). n=4 biological donors. (*P<0.05, **P<0.001).