Figure 4: Overexpression of miR-24 inhibits early Aa-LPS induced IL-10 production in resting but not classically primed macrophages. IL-10 levels in the culture supernatant of macrophages trans-fected with miR-24 mimic, inhibitor, control mimic (all at 50 nM), or positive control (untransfect-ed) for 18 h, primed with IFN-γ (10 ng/ml), IFN-γ plus TNF-α (1 ng/ml), IFN-γ+TNF-β (1 ng/ml), IFN-γ plus IL-17A (10 ng/ml), or positive control (no cytokine priming) for 18 h, and stimulated with Aa-LPS (100 ng/ml) for 96 h, was assayed by ELISA. A, B, C=Mean values, error bars (standard deviation), and significance calculated by ANOVA). n=4 biological donors. (*P<0.05, **P<0.001).