Figure 5: Overexpression of miR-24 in macrophages decreases PI 3- kinase p110δ subunit expres-sion. Macrophages were transfected with miR-24 mimic, inhibitor, control mimic (all at 50 nM), or positive control (untransfected) for 48 h, and expression of p110δ and GAPDH was assessed by western blot analysis. ImageJ software (http://rsbweb.nih.gov/ij/) was used to quantify the results. The values for each lane were normalised with respect to endogenous control GAPDH. Images are representative of 3 biological donors.