Figure 5: Silencing Nrf2 RNA skews macrophages toward the M2 phenotype. NR8383 cells were transfected with either silencing RNA to Nrf2 (si-Nrf2) or with a control (scrambled) silencing RNA (si-Ctrl). Twenty-four hours after transfection, gene expression of Arg1 and iNOS were assessed by RT-PCR. Cells transfected with si- Nrf2 were significantly skewed toward the M2 phenotype, as shown by an elevated Arg1/iNOS ratio. Data presented as a ratio of fold change, mean ± SEM, n=5, *p<0.05.