| Host-response proteins identified from B. mori |
Accession number |
Total length (amino acids) |
Number of fully conserved substitutions |
Number of conserved substitutions |
Number of semiconserved substitutions |
Total substitutions (%) |
Amino acid replaced at position* |
Conserved domains at which substitution occurred** |
Functions of the conserved domains** |
| Heat shock protein 70 |
ACL36370 |
658 |
354 |
123 |
48 |
525 (79.78%) |
---- |
---- |
|
| Chaperonin subunit 4 delta |
NP_001040107 |
537 |
263 |
101 |
28 |
392
(72.99%) |
p.Thr217Ala p.Ile283Met p.Ala436Ser |
T Complex protein 1
(cd03338) |
To sequester non-native proteins inside their central cavity and promote folding |
| SERPIN-4 |
ACZ81437 |
410 |
27 |
47 |
18 |
92 (22.44%) |
p.Ile143Leu p.Leu297Met |
SERPIN
(cd00172) |
Serine protease inhibitors which regulate coagulation cascades. |
| Paralytic peptide precursor |
NP_001036883 |
131 |
21 |
5 |
17 |
43 (32.82%) |
----- |
----- |
------ |
| Prophenoloxidase activating Enzyme |
AAL31707 |
386 |
79 |
40 |
21 |
140
36.27% |
p.Val229Ala |
Trypsin – like Serine protease
(cd00190) |
Serine protease |
| Prophenoloxidase-2 |
AAG09303 |
693 |
185 |
142 |
44 |
371 (53.53%) |
p.Ile98Leu p.Trp214Tyr p.Asp281Ala p.Asp306Glu p.Asp314Glu p.Phe582Tyr |
Hemocyanin
(Pfam00372) |
Hemocyanins and insect larval storage proteins |
| Aromatic-L-amino-acid decarboxylase
(dopa decarboxylase) |
NP_001037174 |
478 |
230 |
96 |
20 |
346 (72.38%) |
p.Lys205Arg p.Arg207Lys p.Ala250Thr p.Lys359Glu p.Arg410Lys |
DOPA decarboxylase family
(cd06450) |
Catalyzes decarboxylation of tyrosine. |
| Spatzle-1 precursor |
NP_001108066 |
277 |
11 |
10 |
05 |
26 (9.38%) |
----- |
-- |
-- |
| 18 Wheeler precursor
(Toll7-2) |
NP_001116821
|
1295 |
185 |
78 |
34 |
297 (22.93%) |
p.Leu240Met p.Leu293lIe p.Ile327Val p.Gln492Leu p.Ala1044Val p.Glu1137Asp |
Amino acids up to the position 492 are in Leucine rich repeats (Pfam13855).
Alanine at 1044 and Glutamine at 1137 are in the Toll/interleukin-1 receptor (TIR) homology domain (Pfam01582) |
TIR is an intracellular signalling domain that mediates protein-protein interactions between the Toll-like receptors and signal-transduction components |
| Embryonic polarity protein dorsal isoform B |
NP_001036896 |
572 |
99 |
50 |
25 |
174 (30.42%) |
p.Ile90Leu
p.Arg128Lys
p.Ile301Val |
Isoleucine and arginine are substituted in the N-terminal sub-domain of the Rel homology domain (RHD; cd07887) whereas Isoleucine at 301 is substituted in the IPT domain of Dorsal (cd01177). |
RHD is transcription factors, which play roles in mediating innate immunity.
Immunoglobulin-like fold, Plexins, Transcription factors (IPT) domain is part of NF kappa B transcription factor activated by stressful conditions |
| Nuclear factor NF-kappa-B p110 subunit isoform 1 (Relish/BmRel)
|
NP_001095935 |
937 |
75 |
58 |
45 |
178 (18.99%) |
p.Asp134Glu
p.Asn194Asp
p.Asn224Ser
p.Thr230Ser
p.Val670Ala |
Substitution upto 224th position is in N-terminal sub-domain of the Rel homology domain (RHD) (cd07884). Valine substitution is at 670 in the Transient Receptor Potential Ca2+ Channel (TIGR00870) or in the ankyrin repeats (COG0666) |
RHD domains are transcription factors that mediate innate immunity.
TIGR00870 are members of the family that mediate entry of extracellular Ca2+ into cells in response to depletion of intracellular Ca2+ .
Ankyrin repeats associated with signal transduction. |
| Cactus |
BAI67121 |
326 |
24 |
31 |
130 |
185 (56.75%) |
p.Ser23Thr |
Ankyrin repeat protein (PHA02874) |
Associated with signal transduction |
| Notch homolog |
NP_001157370 |
2463 |
173 |
47 |
25 |
245 (9.95%) |
p.Leu622Val p.Asp742Asn p.Thr847Ser |
Calcium binding EGF – like domain (smart00179; cd00054) |
Calcium binding site |
| Chitinase isoform 3 precursor
|
NP_001166832 |
544 |
185 |
78 |
34 |
297 (54.59%) |
p.Arg173Lys p.Pro404Ala p.Thr410Ala |
Substitution at 173 is in the catalytically inactive chitolectin-chitotriosidase (cd02872). |
The domain includes a large number of catalytically inactive chitinase-like lectins |
| Beta-N-acetylglucosaminidase |
BAJ20189 |
633 |
76 |
66 |
25 |
167 (26.38%) |
p.Leu140Ile p.Ala343Thr p.Trp351Phe |
The substitutions are in the domain beta-acetyl hexosaminidase and glycosyl hydrolase (cd06562; Pfam14845 & 00728; COG3525) |
Beta-N-acetylhexosaminidases catalyze the removal of beta-1,4-linked N-acetyl-D-hexosamine residues.
Glycosyl hydrolase (GH) family 20 is a catalytic domain with a triose phosphate isomerase (TIM) barrel fold that catalyse GH reactions |
| Autophagy 5-like |
NP_001135959 |
264 |
48 |
68 |
16 |
132 (50%) |
p.Gly211Asn p.Leu219Met |
Autophagy protein 5 (Pfam04106) |
Apg5 is directly required for the import of aminopeptidase I via the cytoplasm-to-vacuole targeting pathway. |
| Apoptosis-inducing factor |
NP_001189462 |
597 |
85 |
101 |
47 |
233 (39.03%) |
p.Gly93Ala p.Phe408Val p.Pro442Ala p.Phe449Tyr |
Phenylalanine substitution at 408th position is in pyridine nucleotide – disulphide oxidoreductase (Pfam07992).
Substitution at 442 and 449 are in the domain Apoptosis inducing factor (AIF; Pfam14721). |
Class I and class II oxidoreductases and also NADH oxidases and peroxidases.
AIF is a bifunctional mitochondrial flavoprotein critical for energy metabolism and induction of caspase-independent apoptosis |
| Caspase Nc
|
NP_001182396 |
438 |
21 |
34 |
26 |
81 (18.49) |
p.Leu297Phe p.Asp359Asn |
Caspase domain, interleukin-1 beta converting enzyme (ICE) homologues (cd00032’ smart 00115; pfam006560 |
Cysteine-dependent aspartate-directed proteases that mediate programmed cell death (apoptosis) |
| Trehalase-2 |
BAE45249 |
642 |
168 |
99 |
47 |
314 (48.91%) |
p.Asn148Ser p.Val161Phe p.Phe199Leu p.Val391Leu |
Trehalase (pfam 01204) |
It is known to recycle trehalose to glucose and protects of proteins and membranes against a variety of stresses |
