Immune/ Host response protein Accession no. Transmembrane segment Position in sequence Start Stop Length Signal peptide Cleavage site Heat shock protein 70 ACL36370 235 251 17 1 - 20 20 and 21 VCA-DD Chaperonin subunit 4 delta NP_001040107 109 118 10 Not predicted   Serpin-4 ACZ81437 52 64 13 Not predicted   Paralytic peptide precursor NP_001036883 9 22 14 1 - 23 23 and 24 VNA-GV Prophenol oxidase activating factor 3 AAL31707 18 31 14 1 - 24 24 and 25 VVG-QK DOPA decarboxylase NP_001037174 97 113 17 Not predicted   Chitinase isoform 3 precursor NP_001166832 16 31 16 1 - 20 20 and 21 VQC-AD β-N-acetylglucosaminidase BAJ20189 32 45 14 Not predicted   Spatzle-1 precursor NP_001108066 8 20 13 1 - 18 18 and 19 ISA-YK 18 wheeler precursor (BmToll7) NP_001116821 8 23 16 1 - 17 17 and 18 GWT-LM 316 327 12 529 539 11 Apoptosis inducing factor NP_001189462 33 49 17 Not predicted   87 97 11 261 270 10 354 364 11 Autophagy 5 like NP_001135959 46 50 5 Not predicted   Nedd2-like caspase NP_001182396 195 206 12 Not predicted   Notch homolog NP_001157370 25 40 16 Not predicted   348 357 10 544 553 10 1538 1547 10 1679 1700 22 1847 1857 11 1934 1946 13 1964 1974 11 Trehalase-2 BAE45249 14 30 17 1 - 18   18 and 19 VVA-DR   591 615 25 Cactus BAI67121 138 156 19 Not predicted   145 154 10   Dorsal NP_001036896 212 216 5 Not predicted   Relish NP_001095935 746 757 12 Not predicted   924 935 12

Note: Immune proteins without transmembrane region and Signal peptides were not mentioned; Transmembrane region was predicted by DAS (Dense Alignment Surface method; Cserzo et al. 1997); Signal peptides were predicted by SignalP 4.0 (Petersen et al. 2011).

Table 3: Potential transmembrane region and Signal peptides from immune and host response proteins induced in fifth instar larva of B. mori through infection by E. bombycis.