Figure 5: Estrogen receptor determines innate T-cell response in the heart. Female C57Bl/6, ERαKO and ERβKO mice were infected with CVB3 and killed 7 days later. Hearts were removed and infiltrating inflammatory cells were recovered and evaluated for natural killer T (NKT) cells using PE-mCD1d tetramer and FITC-anti- TCRβ. T-cells expressing the Vγ4 T-cell receptor (TCR) were identified by labeling cells with PE-anti-Vγ4 and FITC-anti-CD69. Summary of NKT and Vγ4+ cells in the different infected mouse strains is given in (A). Representative flow diagrams for NKT (B) and Vγ4+ (C) cells are provided. Results are given as mean ± SEM from 5-6 mice/group. * and **Significantly different than C57Bl/6 mice at p<0.05 and p<0.01, respectively.