Serum sample GTI-Screening
Class I / II ELISA
Luminex (SD)
Class I / Class II
ELISA-Crossm.
Class I / Class II
06/2010 & neg./neg. n.d./n.d. n.d./n.d.
08/2010 $ neg./neg. neg./neg.
(PRA=0%)
neg./neg. (AMS)
07/2011 * neg./neg. n.d./n.d. n.d./n.d.
08/2012 * neg./neg. neg./neg.
(PRA=0%)
n.d./n.d.
08/2013 * neg./neg. n.d./n.d. n.d./n.d.
11/2014 * neg./neg. neg./neg.
(PRA=0%)
n.d./n.d.
03/2015 # neg./neg. neg./neg.
(PRA=0%)
neg./neg. (AbCr.)
for 03/2015 additionally:
LAT 1HD (anti-HLA class I Single Antigen ELISA): negative
LAT 1288 (anti-HLA class I/II Single Donor ELISA): negative
n.d.: not done; neg.: negative; pos.: positive; PRA%: Panel Reactive Antibodies %; SD: Single Donor Resolution; &: analyses for entering the waiting list; $: analyses at the date of the transplantation; *: routinely performed post-trans-plantation analyses; #: analyses at the date of the clinically proven rejection episode; (AMS), (AbCr.): after its discontinuation in 2013 the AMS-crossmatch ELISA was replaced by the highly modified AbCross-crossmatch ELISA
Table 1: Results of different antibody detection and specification analyses in comparison with crossmatch-ELISA outcomes most probably excluding an involvement of anti-HLA DSA in a rejection episode of the 48-year old heart recipient.