Purification step Volume (mL) Proteins (mg/mL) HAU/mL a Rabbit erythrocytes Total HAUa Rabbit erythrocytes SAb Rabbit erythrocytes Purification fold w.r.t. Rabbit erythrocytes Yield percentage w.r.t. Rabbit erythrocytes
Crude extract 100 9.5 2560 256000 269.47 1 100
ASP Fraction 22 6.0 5120 112640 853.33 3.1 40
ACF Fraction 15 1.84 10240 153600 5565.21 20.65 60
IEC Fraction 5 0.900 20480 102400 22755.55 84.44 40
Table 1: Purification chart of Zizyphus oenoplia seed lectin (ZOSL). Haemagglutination assays were conducted with a suspension of rabbit erythrocytes. It was observed that as the degree of purification increases, the haemagglutination activity also increases. (Haemagglutination assays were conducted with a suspension of rabbit erythrocytes. a) HAU- Hemagglutination Unit, b) SA- Specific activity, ASP fraction- Ammonium sulphate precipitated fraction, ACF fraction- Affinity chromatography fraction, IEC fraction- Ion-exchange chromatography fraction).