Figure 7: Effect of phototherapy on anti-inflammatory cytokine IL-10 in presence on N-acetylcysteine. Supernatant from U937 cells cultured with LPS (1 μg.mL-1) and treated or not with phototherapy (4.5 J/cm2) were collected in order to analyze the protein concentration of IL-10. Before the exposure to LPS or LPS plus laser, the U937 cells were treated with 20 mM N-acetylcysteine (NAC). Twelve hours after the U937 cells stimulation with LPS, the IL-10 protein concentration was determined by Enzyme Linked Immuno Sorbent Assay (ELISA) using kit. Each bar represents mean ± S.D. of three independent experiments. Statistical differences were considered when p<0.05.