Figure 9: Effect of phototherapy on anti-inflammatory cytokine IL-10 in presence of mithramycin. U937 cells were preincubated with mithramycin (100 nM), a Sp1 specific inhibitor, and were activated with LPS (1 μg.mL-1) or LPS plus phototherapy (4.5 J/ cm2). Twelve hours after the LPS exposure, the U937 cells supernatant was assayed by ELISA to determine the production of IL-10. Each bar represents mean ± S.D. of three independent experiments. Statistical differences were considered when p<0.05.