Figure 10: Effect of phototherapy on LPS-induced NF-κB activation. U937 cells cultured with LPS (1 μg.mL-1) during three hours and treated or not with phototherapy (4.5 J/cm2) were lysed and disrupted one hour after the LPS addition. U937 cells were incubated with NAC (20 mM) one hour before the addition of LPS in the culture medium. Twelve hours after LPS addition, the intracellular content of NF-κB protein (10A) was determined using NF-κB ELISA kit and the phosphorylated NF-κB p65 (RelA) (10B) concentration was determined using a calorimetric cell-based ELISA. Each bar represents mean ± S.D. of three independent experiments. Statistical differences were considered when p<0.05.