Figure 4: Effects of the treatment regimen on bladder function (urodynamics); A. Representative bladder pressure tracings from a single rat from each of the 4 experimental groups indicated. Included for each are the initial tracing taken just before the rat was entered into the experimental regimen and one taken at the end, 24 h after bladder wall injection. Not shown is the scale tracing which indicate the voided amounts each time there is a major peak in bladder pressure (voiding pressure); B-E. Quantitation of various urodynamics parameters recorded from rats in each of the indicated experimental groups. Initial tracings were made and each of the parameters quantitated as described in the Methods section. Rats were then subjected to the dosing regimen with a final urodynamics tracing made at the end and quantitated. Results for any given parameter were then calculated as the % of the initial value for that individual rat. The percentages of all individual rats in a given group were then averaged and the bars in the figure represent the mean ± SEM of those percentages; B. Void Volume; C. Intercontraction Interval (time between two voiding contractions). D. Voiding Pressure (maximum pressure associated with a void); E. Threshold Pressure (pressure just before the initiation of a voiding contraction). n=4 for vehicle-treated saline-injected, 5 for Glytreated LPS-injected, 6 for Gly-treated saline-injected and 11 for vehicle-treated LPS-injected. Asterisks indicated significant differences (P<0.05) between the indicated bars.