Figure 5: More Interferon-γ (IFN- γ) was expressed in human T lymphocytes expanded in ZYX Bioreactor. Cells were collected on day 6 and stained with IFN-γ-FITC and CD3-PE. The percentage of IFN-γ and CD3 double positive/ CD3 positive cells was obtained from flow cytometry analysis. The significantly more T cells in ZYX Bioreactor culture expressed INF-γ than static culture (P<0.01, n=6).