Samples Genes Depth of Allele 1 Closest HLA genotype Allele 1 Depth of Allele 2 Closest HLA genotype Allele 2 S1 (Heterozygous) HLA-A 183 A*01:01:01 223 A*68:01:02:01 HLA-B 127 B*37:01:01 110 B*27:05:02 HLA-C 185 C*02:02:02 189 C*06:02:01:01 HLA-DRB1 271 DRB1*10:01:01 103 DRB1*13:01:01 HLA-DQB1 123 DQB1*05:01:01 117 DQB1*06:03:01 S2 (Heterozygous) HLA-A 255 A*02:11:01 152 A*30:01:01 HLA-B 92 B*07:02:01 87 B*50:01:01 HLA-C 165 C*03:04:01:02 189 C*06:02:01:02 HLA-DRB1 205 DRB1*07:01:01 194 DRB1*15:06:01 HLA-DQB1 105 DQB1*02:02:01 139 DQB1*05:02:01 S3 (Heterozygous) HLA-A 500 A*26:01:01 242 A*30:01:01 HLA-B 50 B*50:01:01 92 B*53:01:01 HLA-C 227 C*04:01:01 192 C*06:02:01:02 HLA-DRB1 36 DRB1*15:06:01 24 DRB1*15:03:01 HLA-DQB1 101 DQB1*05:02:01 179 DQB1*06:02:01 S4 (Heterozygous) HLA-A 224 A*01:01:01 164 A*03:01:01:01 HLA-B 179 B*37:01:01 125 B*40:06:01:01 HLA-C 167 C*03:04:01:02 198 C*06:02:01:01 HLA-DRB1 500 DRB1*10:01:01 51 low quality reads HLA-DQB1 45 DQB1*04:02:01 224 DQB1*05:01:01 S5 (Heterozygous) HLA-A 296 A*11:01:01:01 234 A*24:02:01:01 HLA-B 70 B*35:01:01 132 B*56:01:01 HLA-C 457 C*04:01:01:01 425 C*07:04:01 HLA-DRB1 500 DRB1*14:04 279 DRB1*15:02:01 HLA-DQB1 267 DQB1*05:03:01 362 DQB1*06:01:01 S6 (Heterozygous) HLA-A 264 A*02:11:01 - No reads HLA-B 184 B*07:02:01 184 B*18:01:01:02 HLA-C 240 C*07:01:01 151 C*07:02:01:03 HLA-DRB1 500 DRB1*03:01:01 134 DRB1*04:04:01 HLA-DQB1 222 DQB1*03:02:01 201 DQB1*02:01:01 S7 (Homozygous) HLA-A 500 A*01:01:01:01     HLA-B 66 B*37:01:01     HLA-C 500 C*06:02:01:01     HLA-DRB1 500 DRB1*07:01:01:01     HLA-DQB1 152 DQB1*02:02:01     S8 (Homozygous) HLA-A 500 A*01:01:01:01     HLA-B 256 B*37:01:01     HLA-C 500 C*06:02:01:01     HLA-DRB1 104 DRB1*07:01:01     HLA-DQB1 441 DQB1*02:02:01

Table 4: Comparative HLA alleles analyses from SSP/SSOP, 454 sequencing and PacBio sequencing.