adoptive
Figure 8: Elevated HA-specific IgG production by adoptive transfer in vitro expanded lymphocytes. 107 expanded cells and 5 × 105 unexpanded cells were respectively injected into pre-immunized old mice through tail vein. HA-specific IgG levels were assessed by ELISA in which the positive serum was used as a calibrator with arbitrary unit. The adoptive transfer with expanded mononuclear cells (Figure 8a), expanded CD4+ cells (Figure 8b) and expanded CD20+ B cells (Figure 8c) all elevated HA-specific IgG production (P<0.01 at days 2 and 4, P<0.05 at day 6). In contrast, only the unexpanded CD4+ T cells (Figure 8b) from young mice slightly increased the HA-specific IgG (P<0.05 at days 2 and 4 only). The mice received the expanded cells from young mice showed peak antibody response on day 2, which is significantly higher (P<0.01) than the peak response (day 4) of the mice received the expanded cells from old mice and significantly higher (P<0.01) than the antibody level of the mice received the expanded cells from old mice at the same time point (day 2).