Figure 3: Calpastatin degradation is induced by TGF-ß, blocked by IL-5 and precedes calpain cleavage and activation. (A) Immunoblot analysis for calpastatin and calpain-1 in PBEos treated as shown. (B) Immunoprecipitation (IP) and immunoblot analysis of PBEos treated as shown for 24h. IP’s analyzed as shown and representative of at least 3 independent experiments from different donors.