Figure 3: Calpastatin degradation is induced by TGF-, blocked by IL-5 and precedes calpain cleavage and activation.
(A) Immunoblot analysis for calpastatin and calpain-1 in PBEos treated as shown.
(B) Immunoprecipitation (IP) and immunoblot analysis of PBEos treated as shown for 24h. IPs analyzed as shown and representative of at least 3 independent experiments from different donors.