Figure 4: TGF-1 inhibits Akt phosphorylation induced by IL-5 signaling.
(A) 72 hr viability of PBEos left untreated (0), treated as shown with or without SB431542 (SB).
(B, C and D) Immunoblots of PBEos treated with SB, LY294002 (LY) or Akt Inhibitor IV (AktI).
(E) and (F) Viability of PBEos treated as shown and presented as a percentage of viability at time 0. **, p< 0.03 (one-way ANOVA). All blots are representative of at least 3 independent experiments with different donors and data are expressed as the mean SEM.