Figure 5: PI-3-K/Akt (PKB) are upstream of calpastatin/calpain.
(A) Eosinophils were left untreated, or treated as shown for 72h. Cell viability was determined by FACS and presented as a percentage of viability at time 0. **, P< 0.01 (one-way ANOVA).
(B) Immunoblot for calpain-1 and calpastatin after 20 hr incubation under the conditions shown. All blots are representative of at least 3 independent experiments with different donors and data are expressed as the mean SEM..