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Figure 5: PI-3-K/Akt (PKB) are upstream of calpastatin/calpain. (A) Eosinophils were left untreated, or treated as shown for 72h. Cell viability was determined by FACS and presented as a percentage of viability at time 0. **, P< 0.01 (one-way ANOVA). (B) Immunoblot for calpain-1 and calpastatin after 20 hr incubation under the conditions shown. All blots are representative of at least 3 independent experiments with different donors and data are expressed as the mean ± SEM.. |