**Figure 1:** **Enumeration of blood MDC and PDC.** **(A)** Gating strategy for defining multiple blood MDC subsets and PDC. Left dot plot: events shown are gated upon
CD45^{+} WBC. Middle dot plot: total CD11c^{+}MDC (Gate 2) and CD123^{+}PDC (Gate 3) are gated upon Lin^{-}HLA-DR^{+} cells (Gate 1). Right dot plot: CD16^{-}CD141^{+}MDC (Gate
4), CD16^{+}Cd141^{-}MDC (Gate 5) and CD16^{-}CD141^{-}MDC (Gate 6) are gated upon CD11c^{+}MDC (Gate 2). **(B)** Scatter plot of the absolute number of total CD11c^{+}MDC
(median), CD16^{+}MDC (median), CD16^{-}CD141^{-}MDC (median), CD16^{-}CD141^{+}MDC (mean) and CD123^{+}PDC (mean). Absolute numbers of CD16^{+}MDC and CD123^{+}PDC
correlated with age in T1D children, siblings and controls (Spearman P and r value). *, P < 0.05; **, P < 0.01. |