Figure 2: Enumeration of blood monocytes. (A) Scatter plot of the absolute number of monocytes obtained by full blood count (mean). Absolute number of monocytes obtained by full blood count correlated with age in T1D children, siblings and controls (Spearman P and r value). **, P < 0.01; ***, P < 0.001. (B) Dot plot: gating strategy used to define blood monocyte subsets. The CD14+CD16- (Gate 1) and CD14+CD16+ (Gate 2) monocyte subsets are gated upon CD45+ WBC. Scatter plot of the absolute numbers of total CD14+ monocytes (Gate 1+2, median), CD14+CD16+ monocytes (Gate 2, median) and CD14+CD16- monocyes (Gate 1, mean). Absolute numbers of total monocytes and CD14+CD16+ monocytes correlated with age in T1D children, siblings and controls (Spearman P and r value). *, P < 0.05; ***, P < 0.001.