Figure 2: CD4+ T-cells dynamics in response to Mtb challenge.
A. Frequency of CD4+ T-cells in lymphocytes and percentage of IL-7Rα+ CD4+ T-cells after BCG vaccination (and control animals).
B. Frequency of CD45RA/CCR7 compartments (as described in Figure 1) in CD4+ T-cells after BCG or rBCG vaccination followed by adenoviral boots (and control animals). Vaccinated animals showed a trend of decreased frequency of CD45RA+CCR7+ T-cells (p<0.04) and increased frequency of CD45RA-CCR7+/- T-cells (p<0.04 in the rBCG vaccinated group) 2 weeks after Mtb challenge. This was absent in control animals. CD45RA+CCR7+ T-cells constituted the most frequent subset in all NHP groups 20 weeks after Mtb challenge. Note, that from 15 weeks after Mtb challenge, 2 animals in the BCG-vaccinated group, 3 animals in the control group, and from 20 weeks after Mtb challenge, 2 animals in the BCG-vaccinated group were available for analysis since NHPs had to be sacrificed due to TB-associated symptoms.