Figure 2: The cross-reactivity of isolated antibodies as established in the competitive ELISA. (A) Sample 1. The inhibition of the binding of IgG to the adsorbed 10% ТFβ-pAA conjugate by soluble conjugates: 10% ТFβ-pAA (•), 10% TF-pAA (■) and 20% GA1-PAA (▲, IC50 = 2.05 μM). The inhibition of the antibody binding to the pAA-carrier by the soluble 20% GA1-PAA (△, IC50 = 1.48 M). (B) The inhibition of the binding of IgG (sample 5) to the adsorbed 10% ТFβ-РАА conjugate by soluble conjugates: 10% ТFβ-РАА (○), pAA (□) and 20% Gb5tri-PAA (▽). The inhibition of the binding of IgM (sample 7) to the adsorbed 10% ТFβ-РАА conjugate by soluble conjugates: 10% ТFβ-РАА (•), 20% GA1-PAA (▲) and 20% Gb5tri-PAA (▼). (C) The inhibition of the binding of IgG (sample 2) to the adsorbed 10% ТFβ-РАА conjugate by soluble conjugates: 10% ТFβ-РАА (•), 10% TF-РАА (■), 20% GA1-PAA (▲) and 20% Gb5tri-PAA (▼), free TF-sp (□) and free Gb5tri (▽), and GA1 glycolipid (○). (D) The effect of the soluble pAA carrier on the IgG antibodies of sample 2: the inhibition of the antibody binding to the adsorbed 10% ТFβ-pАА (•) and 10% ТFβ-РАА (○) conjugates.