Conjugate

Sample and key ligand a

MAb TF1 IgM

RPA GA1 IgG

1 TFb IgG (KO)

2 TFc IgG (KO)

3 TFb IgG (AM)

4 Tnb IgG (DM)

5 TFc IgG (DM)

6 TFb IgG (KT)

7 TFc IgM (RA)

10% TF-pAA

100

100

100

399

100

100

100

100

100

10% TFβ-pAA

677

499

207

446

88

173

242

6

47

pAA

218

297

190

350

430

171

0

0

12

TFmax-pAA

0

0

NT

0

NT

27

10

139

NT

10% TF-PAA

2

4

0

0

9

6

74

28

NT

10% TFβ-PAA

10

1381

2

12

380

9

157

3

11

20% TF-PAA

8

88

0

0

56

57

413

197

NT

20% GA1-PAA

31

83

4

122

18

14

91

1

600

20% GA2-PAA

0

0

0

0

0

0

0

NT

0

20% Tn-PAA

0

7

11

100

0

0

0

NT

NT

20% Gb5tri-PAA

0

843

6

0

71

109

214

27

54

AG

0

175

5

NT

0

189

86

222

NT


a The key ligand is a glycan that was used in the isolation of antibodies. The reactivity of antibodies to key-ligand conjugates was taken as 100%.
b Antibodies isolated on PAA sorbents.
c Antibodies isolated on the TF-sp-sorbents.
NT – not tested.
Table 3: The reactivity of antibodies to glycoconjugates (Atest minus Acontrol in %, indirect ELISA).