On day 0, 3.2 mg anti-collagen-II antibody mix was injected i.p. in DBA/J1 mice to induce collagen antibody induced arthritis (CAIA), followed by an i.p. injection containing 25 μg LPS on day 3, and RhmAb2.102 or placebo (No Ab) i.p. injection on day 7 (1 mg/mouse). Shown are the means ± SEM (n=5). Mice were scored daily for signs of inflammation in their paws. Scoring was performed according to the method outlined in Table 1. (A) Mean arthritis score of all 4 paws of all mice per group. (B) Mean arthritis score of the right hind paws of all mice per group. Right hind paws were used for histological analysis (Figures 3 and 4).
Figure 2: Therapeutic RhmAb2.102 treatment.