On day 0, 3.2 mg anti-collagen-II antibody mix was injected i.p. in DBA/J1 mice to induce CAIA, followed by an i.p. injection containing 25 μg LPS on day 3, and RhmAb2.102 or placebo i.p. injection on day 7 (1 mg/mouse). Panel A shows that macroscopic arthritis score in the right hind paws between experimental groups was similar on day 35. Paraffin sections were made from right hind paws and consecutive sections were stained with either Haematoxylin-Eosin or Safranin O staining. Sections were scored for the following parameters: cartilage erosion (B), bone erosion (C), inflammatory cell influx (D), cartilage PG depletion (E) and chondrocyte death (F). All parameters were scored on a scale from 0-3, with 0 meaning no pathological signs present, and 3 indicating maximum pathological signs.
